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high-sensitivity flow cytometry for nanoparticle tracking analysis (nta)  (NanoFCM Inc)

 
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    NanoFCM Inc high-sensitivity flow cytometry for nanoparticle tracking analysis (nta)
    TAMs from HNSCC predominantly exhibit the M2 phenotype. A The morphological changes that occur when M0 macrophages were induced into TAMs by SCC25/CAL27-conditioned medium. The blue arrow indicated the emergence of pseudopodia in TAMs. B RT-qPCR was used to detect the mRNA levels of M2 phenotype markers ( FIZZ-1 , ARG-1 ) in TAMs. C Western blot was used to detect the protein levels of the M2 phenotype markers in TAMs (CD163, CD206). D Representative images of IF staining for CD163, CD206 and F-actin in macrophages induced by HNSCC conditioned medium, Scale bars = 50 μm. E Expression of classical M2 macrophage markers (CD206 and CD163) was evaluated by flow cytometry. F Western blot was performed to detect the expression of positive markers (TSG101, CD63, CD9) and negative marker (Calnexin) in the TAM-EVs. G Electron microscopy images of EVs were isolated from M0/TAM-CM. H <t>Nanoparticle</t> tracking analysis (NTA) of M0/TAM-sEVs. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
    High Sensitivity Flow Cytometry For Nanoparticle Tracking Analysis (Nta), supplied by NanoFCM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high-sensitivity flow cytometry for nanoparticle tracking analysis (nta)/product/NanoFCM Inc
    Average 90 stars, based on 1 article reviews
    high-sensitivity flow cytometry for nanoparticle tracking analysis (nta) - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma"

    Article Title: Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma

    Journal: Cellular and Molecular Life Sciences

    doi: 10.1007/s00018-024-05210-6

    TAMs from HNSCC predominantly exhibit the M2 phenotype. A The morphological changes that occur when M0 macrophages were induced into TAMs by SCC25/CAL27-conditioned medium. The blue arrow indicated the emergence of pseudopodia in TAMs. B RT-qPCR was used to detect the mRNA levels of M2 phenotype markers ( FIZZ-1 , ARG-1 ) in TAMs. C Western blot was used to detect the protein levels of the M2 phenotype markers in TAMs (CD163, CD206). D Representative images of IF staining for CD163, CD206 and F-actin in macrophages induced by HNSCC conditioned medium, Scale bars = 50 μm. E Expression of classical M2 macrophage markers (CD206 and CD163) was evaluated by flow cytometry. F Western blot was performed to detect the expression of positive markers (TSG101, CD63, CD9) and negative marker (Calnexin) in the TAM-EVs. G Electron microscopy images of EVs were isolated from M0/TAM-CM. H Nanoparticle tracking analysis (NTA) of M0/TAM-sEVs. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
    Figure Legend Snippet: TAMs from HNSCC predominantly exhibit the M2 phenotype. A The morphological changes that occur when M0 macrophages were induced into TAMs by SCC25/CAL27-conditioned medium. The blue arrow indicated the emergence of pseudopodia in TAMs. B RT-qPCR was used to detect the mRNA levels of M2 phenotype markers ( FIZZ-1 , ARG-1 ) in TAMs. C Western blot was used to detect the protein levels of the M2 phenotype markers in TAMs (CD163, CD206). D Representative images of IF staining for CD163, CD206 and F-actin in macrophages induced by HNSCC conditioned medium, Scale bars = 50 μm. E Expression of classical M2 macrophage markers (CD206 and CD163) was evaluated by flow cytometry. F Western blot was performed to detect the expression of positive markers (TSG101, CD63, CD9) and negative marker (Calnexin) in the TAM-EVs. G Electron microscopy images of EVs were isolated from M0/TAM-CM. H Nanoparticle tracking analysis (NTA) of M0/TAM-sEVs. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Techniques Used: Quantitative RT-PCR, Western Blot, Staining, Expressing, Flow Cytometry, Marker, Electron Microscopy, Isolation



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    high-sensitivity flow cytometry for nanoparticle tracking analysis (nta)  (NanoFCM Inc)
    90
    NanoFCM Inc high-sensitivity flow cytometry for nanoparticle tracking analysis (nta)
    TAMs from HNSCC predominantly exhibit the M2 phenotype. A The morphological changes that occur when M0 macrophages were induced into TAMs by SCC25/CAL27-conditioned medium. The blue arrow indicated the emergence of pseudopodia in TAMs. B RT-qPCR was used to detect the mRNA levels of M2 phenotype markers ( FIZZ-1 , ARG-1 ) in TAMs. C Western blot was used to detect the protein levels of the M2 phenotype markers in TAMs (CD163, CD206). D Representative images of IF staining for CD163, CD206 and F-actin in macrophages induced by HNSCC conditioned medium, Scale bars = 50 μm. E Expression of classical M2 macrophage markers (CD206 and CD163) was evaluated by flow cytometry. F Western blot was performed to detect the expression of positive markers (TSG101, CD63, CD9) and negative marker (Calnexin) in the TAM-EVs. G Electron microscopy images of EVs were isolated from M0/TAM-CM. H <t>Nanoparticle</t> tracking analysis (NTA) of M0/TAM-sEVs. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
    High Sensitivity Flow Cytometry For Nanoparticle Tracking Analysis (Nta), supplied by NanoFCM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high-sensitivity flow cytometry for nanoparticle tracking analysis (nta)/product/NanoFCM Inc
    Average 90 stars, based on 1 article reviews
    high-sensitivity flow cytometry for nanoparticle tracking analysis (nta) - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    high sensitivity flow cytometry for nanoparticle tracking analysis (nta)  (NanoFCM Inc)
    90
    NanoFCM Inc high sensitivity flow cytometry for nanoparticle tracking analysis (nta)
    TAMs from HNSCC predominantly exhibit the M2 phenotype. A The morphological changes that occur when M0 macrophages were induced into TAMs by SCC25/CAL27-conditioned medium. The blue arrow indicated the emergence of pseudopodia in TAMs. B RT-qPCR was used to detect the mRNA levels of M2 phenotype markers ( FIZZ-1 , ARG-1 ) in TAMs. C Western blot was used to detect the protein levels of the M2 phenotype markers in TAMs (CD163, CD206). D Representative images of IF staining for CD163, CD206 and F-actin in macrophages induced by HNSCC conditioned medium, Scale bars = 50 μm. E Expression of classical M2 macrophage markers (CD206 and CD163) was evaluated by flow cytometry. F Western blot was performed to detect the expression of positive markers (TSG101, CD63, CD9) and negative marker (Calnexin) in the TAM-EVs. G Electron microscopy images of EVs were isolated from M0/TAM-CM. H <t>Nanoparticle</t> tracking analysis (NTA) of M0/TAM-sEVs. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001
    High Sensitivity Flow Cytometry For Nanoparticle Tracking Analysis (Nta), supplied by NanoFCM Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity flow cytometry for nanoparticle tracking analysis (nta)/product/NanoFCM Inc
    Average 90 stars, based on 1 article reviews
    high sensitivity flow cytometry for nanoparticle tracking analysis (nta) - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    TAMs from HNSCC predominantly exhibit the M2 phenotype. A The morphological changes that occur when M0 macrophages were induced into TAMs by SCC25/CAL27-conditioned medium. The blue arrow indicated the emergence of pseudopodia in TAMs. B RT-qPCR was used to detect the mRNA levels of M2 phenotype markers ( FIZZ-1 , ARG-1 ) in TAMs. C Western blot was used to detect the protein levels of the M2 phenotype markers in TAMs (CD163, CD206). D Representative images of IF staining for CD163, CD206 and F-actin in macrophages induced by HNSCC conditioned medium, Scale bars = 50 μm. E Expression of classical M2 macrophage markers (CD206 and CD163) was evaluated by flow cytometry. F Western blot was performed to detect the expression of positive markers (TSG101, CD63, CD9) and negative marker (Calnexin) in the TAM-EVs. G Electron microscopy images of EVs were isolated from M0/TAM-CM. H Nanoparticle tracking analysis (NTA) of M0/TAM-sEVs. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Cellular and Molecular Life Sciences

    Article Title: Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma

    doi: 10.1007/s00018-024-05210-6

    Figure Lengend Snippet: TAMs from HNSCC predominantly exhibit the M2 phenotype. A The morphological changes that occur when M0 macrophages were induced into TAMs by SCC25/CAL27-conditioned medium. The blue arrow indicated the emergence of pseudopodia in TAMs. B RT-qPCR was used to detect the mRNA levels of M2 phenotype markers ( FIZZ-1 , ARG-1 ) in TAMs. C Western blot was used to detect the protein levels of the M2 phenotype markers in TAMs (CD163, CD206). D Representative images of IF staining for CD163, CD206 and F-actin in macrophages induced by HNSCC conditioned medium, Scale bars = 50 μm. E Expression of classical M2 macrophage markers (CD206 and CD163) was evaluated by flow cytometry. F Western blot was performed to detect the expression of positive markers (TSG101, CD63, CD9) and negative marker (Calnexin) in the TAM-EVs. G Electron microscopy images of EVs were isolated from M0/TAM-CM. H Nanoparticle tracking analysis (NTA) of M0/TAM-sEVs. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: The diameter of the sEV was measured using high-sensitivity flow cytometry for nanoparticle tracking analysis (NTA; Nanofcm, Fujian, China).

    Techniques: Quantitative RT-PCR, Western Blot, Staining, Expressing, Flow Cytometry, Marker, Electron Microscopy, Isolation